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Available at: Singh, P., et al

The high number of potentially active -defensins is remarkable in mammalia, and within the subgroup of Laurasiatheria the Equidae are the only known family expressing -defensin genes

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Then, 10 g of total protein lysate was loaded for SDS-PAGE (4% stacking, 12% separating gel), and proteins were transferred on a PVDF membrane using a tank-blotting system at 30 V overnight